【摘要】 利用甘薯品种栗子香、高自1号和高系14号,对甘薯细胞悬浮培养及有效植株再生进行了研究.将长约0.5mm的茎尖组织培养在添加2.0mg·L^-12,4-D的MS培养基上,诱导得到胚性愈伤组织.将获得的胚性愈伤组织在含有2.0mg·L^-12,4-D的MS液体培养基中进行振荡培养,建立了增殖迅速、分散程度良好的胚性细胞悬浮培养系.悬浮培养24周后,将直径约1.0mm的细胞团转移到添加2.0mg·L^-12,4-D的MS培养基上,增殖成为胚性愈伤组织,并形成体细胞胚.将具有体细胞胚的胚性愈伤组织进一步转移到添加1.0mg·L^-1ABA的MS培养基上时,体细胞胚发芽形成小植株,三个品种的植株再生率均达到100%;悬浮培养37周后,栗子香、高自1号和高系14号的植株再生率分别为45.8%、83.3%和79.2%.更多还原

【Abstract】 Cell suspension cultures and efficient plant regeneration were studied through the use of cvs. Lizixiang, Gaozi No. 1, and Kokei No. 14 in sweet potato, Ipomoea batatas （L. ） Lam. . Shoot tips of about 0. 5 mm in length gave rise to embryogenic calli on MS medium supplemented with 2. 0mg·L^-12,4-D. The obtained embryogenic calli were used to initiate cell suspension cultures in MS medium containing 2. 0 mg ·L-12,4-D. Rapidly-growing and well-dispersed cell suspension cultures were established. Twenty-four weeks after the initiation, cell aggregates about 1. 0 mm in diameter were transferred to MS agar medium supplemented with 2. 0 mg·L^-12,4-D and formed embryogenic calli with somatic embryos. The embryogenic calli with somatic embryos were further transferred to MS agar medium supplemented with 1. 0 mg ·L^-1ABA, resulting in the germination of somatic embryos. The frequencies of plant regeneration reached 100% in all three cultivars. Thirty-seven weeks after the initiation, the frequency of plant regeneration of Lizixiang, Gaozi No. 1, and Kokei No. 14 was still 45. 8%, 83. 3% > and 79. 2% , respectively.更多还原