节点文献
蜡蚧轮枝菌11个单孢菌株的生物学及其对温室白粉虱致病性的比较和筛选
The Biology of Eleven Verticillium lecanii Isolates Cultured with Monospores and the Comparison and Selection of Their Pathogenicity against Trialeurodes vaporariorum
【摘要】 本文通过对北京地区蜡蚧轮枝菌11个单孢分离菌株的菌落生长、产孢量、分生孢子萌发率以及对温室白粉虱2龄若虫致病性研究比较,结果表明:上述性状特别是产孢量,在11个菌株中均表现有显著差异,但孢子萌发率都较高,达94.01%~9897%(12h)。在同一菌株中这些性状有的表现出一致性,有的则不同。综合比较几种性状,筛选出B3/S1-SUb2和4号两个优良菌株,它们首先具有致病性高(对2龄粉虱若虫的致死率>95.76%,12d)、其次孢子萌发率高(94.24%和98.42%)、产孢量大(5.06和4.38×109抱子/皿)以及菌落生长速率快(3.13和3.00mm/d)的特性,具有实用价值,可为今后生物制剂的生产提供菌株。
【Abstract】 The preliminary study showed that significant differences exist in the mycelial growth,germination rate of conidia and pathogenicity againist the second instar nymphs of Trialeurodes vaporariorum westwood,especially in sporulation yields among eleven Verticillium lecanii (Zimm. ) viegas isolates. These characteristics of the fungus are consistent in some isolates,but different in the others. However conidial germination rate of all isolates were high, ranging from 94. 01 % to 98. 97% (12 h). The isolates B3/S1-Sub22 and 4 were selected based on Comprehensive comparisons in eleven isolates. Both isolates B3/S1-Sub22 and 4 had high pathogenicity (over 95. 76% mortality for the 2nd instar nymphs of whitefly),high germination rate of conidia (94. 24% and 98. 42% rep. ), more sporulation yields (5. 06 and 4. 83×109 spores/dish)and rapid mycelial growth (3. 31 and 3. 00 mm/day).
【Key words】 Verticillium lecanii; isolates; conidia; pathogenicity; Trialeurodes vaporarioum;
- 【文献出处】 中国农业大学学报 ,Journal of China Agricultural University , 编辑部邮箱 ,1996年01期
- 【分类号】S476.12;Q9
- 【被引频次】69
- 【下载频次】154