【摘要】 用ＰＡＲＰ酶抑制剂苯甲酰胺（ＢＡ）处理经ｃ－Ｈａ－Ｔ２４－ｒａｓ活化癌基因转染的ＮＩＨ３Ｔ３细胞得到转化细胞系，结果表明：经苯甲酸胺处理的细胞系，其细胞凝集、血清依赖性、形态特征等均发生了改变，呈现出正常细胞的特征．Ｓｏｕｔｈｅｒｎ杂交结果表明，已整合到细胞基因组中的外源Ｔ２４～ｒａｓ基因发生了丢失．更多还原

【Abstract】 A transformant obtained by transfection of NIH3T3 cells with c-Ha-T24-ras oncogene was treated with benzamide,an inhibitor of PARP enzyme. Some changes including the properties of cell agglutination, serum dependence, morphology for the transformant line were ob served. Southern blot analysis showed that the exogenous c-Ha-T24-ras gene which had been integrated into genomic DNA of recipient cells was deleted.更多还原