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银杏、金钱松PSⅡ颗粒放氧活性及多肽组分的研究
Photosynthetic O 2-Evolving Activity and Polypeptides Composition of PSⅡ Particles From Gymnosperm-Ginkgo and Pseudoiarix
【摘要】 以银杏、金钱松为裸子植物的代表性实验材料对其PSⅡ光合放氧及多肽组分进行了研究,和菠菜相比较,银杏、金钱松PSⅡ放氧活性较低,分别为菠菜的10%和35%左右.对银杏、金钱松的PSⅡ多肽组与分析发现,其多肽组分较复杂,和被子植物菠菜相比较,多肽数量多,且含有分子量为65000、61000、52000的大分子多肽.小分子多肽也存在一定的差异.
【Abstract】 We prepared PSⅡ particles from gymnosperm--Ginkgo and Pseudolarix. The preparation showed O 2-evolution activity of 11.7 and 38.60 O 2·μmol mg -1 Chl h -1 . The O 2-evolution activity were 10% and 35% that of Spinach PSⅡ particles respectively. Densitometer traces of SDS-PAGE indicated Ginkgo PSⅡ particles contained 11 polypeptides and Pseudolarix PSⅡ particles contained 9 polypeptides. The results demonstrated that the high-molecular weight polypeptides in gymnosperm (Ginkgo, Pseudolarix) PSⅡ particles were not found in angiosperm (Spinach) PSⅡ particles.
【Key words】 Ginkgo; Pseudolarix; PSⅡ particles; Polypeptides; O 2-evolution activity;
- 【文献出处】 南京师大学报(自然科学版) ,JOURNAL OF NANJING NORMAL UNIVERSITY(NATURAL SCIENCE EDITION) , 编辑部邮箱 ,1996年03期
- 【分类号】S792.95
- 【被引频次】2
- 【下载频次】65