【摘要】 ＰＣＲ／ＳＳＰ（Ｓｅｑｕｅｎｃｅｓｐｅｃｉｆｉｃｐｒｉｍｅｒ，序列特异性引物）是一种新的基因多态性分析技术，可用于ＨＬＡ复合体及其他具有多态性特点的基因分型。我室于１９９３年建立这种方法，并对正常人、重症肌无力患者、全身性红斑狼疮患者、骨髓移植供／受者进行了ＨＬＡ－ＤＲＢ１、－ＤＱＢ１基因型别分析。与其它ＨＬＡ基因分型方法相比，ＰＣＲ／ＳＳＰ具有简便、快速、准确及重复性好等特点。本文介绍ＰＣＲ／ＳＳＰ技术的原理、方法、特点以及常见问题的处理。更多还原

【Abstract】 PCR amplification with sequence specific primers(PCR/SSP) is a new method used to determine the allelic polymorphism of HLA chass Ⅱ molecules.After the establishment of this novel technique in our workgroup in 1993,HLA DBR1 typing using PCR/SSP were carried out among normal Hubei Chinese Hans,patients with myathemia gravis(MG)and systemic lupus erythematosus(SLE),donors and recipients of organ transplantation.It is demonstrated that the HLA DRB1 typing by PCR/SSP is simple,rapid and acurate,and is characterized with high resolution and good reproducibility,compared with other HLA class Ⅱ typing techniques such as PCR/SSO and PCR/RFLP.The principle,method,charactors of PCR/SSP as well as common trouble shootings were presented in this paper.更多还原