【摘要】 以５－ｂｒｏｍｏ－２－ｄｅｏｘｙｕｒｉｄｉｎｅ标记法检测了ＳＰ２／０细胞的增殖动力学，并与３Ｈ－ＴｄＲ掺入法作了比较，结果表明本法是可靠的，且有很高的特异性。该法较３Ｈ－ＴｄＲ掺入的传统方法简便、敏感、精确，可广泛应用于细胞增殖动力学、肿瘤生物学、遗传学、分子生物学等多个领域。更多还原

【Abstract】 Monoclonal antibody to 5-bromo-2-deoxyuridine was used in conjunction with immunocytochemical procedures to identify cells in which DNA synthesis has occured in the presence of thymidine analogue 5-bromo-2-deoxyuridine,This assay has certain advantages over the method based on radiolabeled thymidine,it is quicker,safer,simpler,more sensitive and more accurate.更多还原