【摘要】 切除羽状柱头后，在小麦花柱切面上滴注含有ＧＵＳ与天花粉蛋白基因的质粒ＤＮＡ。共处理小花１４９５朵，结种子１１９４粒，结实率为７９．９％。随机取收获的烟农１５种子１２５粒，室内萌发，ＰＣＲ扩增证明有３株是含天花粉蛋白基因的转基因植株，转化率为２．４％。ＧＵＳ与天花粉蛋白基因紧密连锁，ＰＣＲ检测结果间接证明ＧＵＳ基因也已导入小麦更多还原

【Abstract】 GUS and TCS (trichosanthin) genes were introduced into wheat via the pollen-tube pathway. Three wheat plants containing foreign TCS gene were detected from 125 plants tested. The transgenic plants were characterized by polymerase chain reaction analysis using two 24bp primers homolous to TCS gene. The result shows that the pollen tube pathway is suitable for genetic transformation of wheat cultivars.更多还原