【摘要】 采用ＳＰ免疫组化法对５２例食管鳞状细胞癌和３０例食管粘膜慢性炎（对照组）进行高危ＨＰＶ１６、１８Ｅ_６和ｐ２１ｒａｓ、ｐ５３癌基因产物的检测。结果表示：鳞癌组中Ｅ_６的阳性率为６７．３１％，与对照组相比差异有极显著性（Ｐ＜０．００１），其中Ｅ_６与ｐ５３呈双阳性者为５５．７７％（２９／５２）、８９．６６％（２６／２９），显示两者阳性着色出现在部分相同区域同一癌细胞核内（似表明Ｅ_６可与ｐ５３结合形成复合物从而导致野生型ｐ５３的降解）。本组ｐ２１ｒａｓ与ｐ５３、ｐ５３与Ｅ_６的阳性表达均具相关性（Ｐ＜０．０５）。提出ＨＰＶ１６、１８感染与本地区食管癌病因学密切相关，Ｅ_６抗体是诊断ＨＰＶ１６、１８感染的良好标记。更多还原

【Abstract】 The expression of HPV16,18 E₆ and p21ras, p53 oncoprotein in 52 cases of esophageal squamous cell carcinoma and 30 cases of chronic esophagitis were examined with immunohistochemistry.The result showed:67.31%of HPV16,18 E₆ was positive in malignant group,but lower expression in the control group（P<0.001）, 55.77%（29/52） were both E₆ and p53 positive and 89. 66%（26/29） stained in the same part of cell nucleus（it seems that binding of p53 with E₆ protein form detectable complexes, which lead to accelerate degradation of wild type p53）. Positive expression of p21ras and p53, p53 and E₆ showed a significant correlation respectively （P<0.05）.It suggested that high risk HPV16,18 infection was closely related to esophageal carcinogenesis, E₆ antibody is an excellent marker in diagnosis of HPV16,18 infection.更多还原