【摘要】 应用抗p53单克隆抗体及LSAB免疫组织化学染色法检测喉鳞癌、喉癌旁组织及声带息肉p53蛋白的表达。结果显示:25例(57％)喉鳞癌呈阳性反应,4例(20％)距肿瘤边缘<0.5cm的喉癌旁组织呈弱阳性反应,20例距肿瘤边缘>2cm的喉癌旁组织及16例声带息肉均为阴性。p53表达与喉癌分型、分期及淋巴结转移不相关,而与病理分化程度密切相关(P<0.01)。表明,抑癌基因p53的突变是肿瘤增生异常活跃的因素之一,在喉癌的发生中可能是一种比较常见的基因改变,并与喉癌的恶性程度有关。更多还原

【Abstract】 The monoclonal antibody to p53 and the LSAB immunohistochemical method were used to detect p53 expression in different sites:visible tumor,nomal mucosa adjacent to the tumor (transitional mucosa),the end of the sample and benign laryngeal nodules. The results showed that 25(57%) of 44 laryngeal squamous cell carcinomas(LSCC) were positive,4 of 20 transitional mu-cosas were also positive,20 cases of the end of the sample and 16 cases of benign lesion were all negative. The differentiation of LSCC was related to the expression of p53 protein(P<0. 01). Our study indecates that the over-expression of p53 is not only related to the LSCC,but also closely related with the malignant level of larynx carcinoma.更多还原