【摘要】 通过对窖泥、大曲、米曲、黑曲及红曲中微生物的分离、筛选，获得两株己酸乙酯合成能力较强的菌株，经初步鉴定，都归于曲霉属．两菌株均在３０℃、ｐＨ为５的皮培养基中培养６０ｈ后产酶最高；Ｍｍ—４菌株的酯化酶在适宜条件下（２％乙醇和０．２５％的己酸作底物，ｐＨ为４．０，３０℃）酶活力可达２２０ｕ／ｇ．更多还原

【Abstract】 Two strains of Aspergillus with higher esterifying enzyme activity were isolated from pit earth and fermenting agent bricks. When they were cultivated in bran medium of initial pH 5. 0 for 60 hours at 30℃,enzyme productions reached their highest. As for the esterifying enzyme of Mm-4 strain,at the optimum conditions (2% alcohol and 0. 25% caproic acid,pH 4. 0,30℃ ),enzyme activity reached its highest of 220 u/g.更多还原