【摘要】 本文研究了正常人极低密度脂蛋白（ｖｅｒｙｌｏｗｄｅｎｓｉｔｙｌｉｐｏｐｒｏｔｅｉｎ，ＶＬＤＬ）两种亚组分，即贫含载脂蛋白Ｅ的ＶＬＤＬ和富含载脂蛋白Ｅ的ＶＬＤＬ对小鼠腹腔巨噬细胞（ｍｏｕｓｅｐｅｒｉｔｏｎｅａｌｍａｃｒｏｐｈａｇｅ，ＭＰＭ）脂质堆积的影响。将贫含载脂蛋白Ｅ的ＶＬＤＬ和富含载脂蛋白Ｅ的ＶＬＤＬ分别与ＭＰＭ温育２４ｈ后，细胞内甘油三酯（ｔｒｉｇｌｙｃｅｒｉｄｅ，ＴＧ）和胆固醇酯（ｃｈｏｌｅｓｔｅｒｙｌｅｓｔｅｒ，ＣＥ）随ＶＬＤＬ浓度的增加而增加（方差分析，Ｐ＜０．０５），且对ＭＰＭ内ＴＧ的增加量以贫含载脂蛋白Ｅ的ＶＬＤＬ居多（Ｐ＜０．０１），而对ＭＰＭ内ＣＥ的增加量两者无显著性差异（Ｐ＞０．０５）。这提示，贫含载脂蛋白Ｅ的ＶＬＤＬ和富合载脂蛋白Ｅ的ＶＬＤＬ均能促进ＭＰＭ内ＴＧ、ＣＥ堆积，从而使巨噬细胞（ｍａｃｒｏｐｈａｇｅ，ＭＰ）转变为泡沫细胞。更多还原

【Abstract】 Aim This study is to investigate the effects of natural very low density lipoprotein (n-VLDL)subfractions (Apo E-poor VLDL and Apo E-rich VLDL)on triglyceride (TG) and cholesteryl ester (CE) accumulation in mouse peritoneal macrophages (MPM).Methods VLDL for subfraction by HeparinSepharose 6B affinity chromatography was obtained from 400 ml of normal human plasma by ultracentrifugation.MPM were incubated with Apo E-poor VDLD or Apo E-rich VLDL at the concentrations of 10～40 mg protein/L DMEM for 24 hours.Results Followed the increased concentrations of VLDL,the accumulation of TG and CE in MPM increased(P<0.05).The TG content in MPM wasmuch higher in Apo E-poor VLDL group than that in Apo E-rich VLDL group (P<0.01). There was little difference in CE content in MPM between both groups (P>0.05).Conclusion These results suggest that both VLDL subfractions can stimulate TG and CE accurnulation in MPM and accelerate the transformation of macrophages into foam cells.更多还原