【摘要】 对栗疫病菌不同毒力菌株产生胞外酶的种类、活性和草酸产量以及草酸对多聚半乳糖醛酸酶水解聚果胶酸钙的影响进行了研究。所有供试菌株均未能检测到淀粉酶活性。栗疫病菌在培养中可分泌漆酶、多聚半乳糖醛酸酶、蛋白酶、纤维素酶和脂酶,但不同毒力菌株产生这些酶的能力不同。总的来说,强毒力菌株均可分泌这些酶,且活性强,但弱毒力菌株的酶活性较弱或不分泌这些酶。菌丝产量和草酸产量分析表明,强毒力菌株的草酸产量明显高于弱毒力菌株。菌丝产量与草酸产量没有相关性。在没有草酸盐存在的条件下,多聚半乳糖醛酸酶不能降解聚果胶酸钙。更多还原

【Abstract】 All isolats of Cryphonectria parasitica did not secrete amylast in cul -ture. Virulent isolates could secrete laccase, polygalacturonase, protease, cellulase and lipase, but hypovirulent isolates did not secrete these extracellular enzymes or the activities of these enzymes were weak in hypovirulent isolates. All isolates produced oxalate in culture, but varying among isolates with different virulence levels. The oxalate productions of virulent isolates were higher markedly than those of hypovirulent isolates. There are no correlation of mycelial dry weights with oxalate production. The polygalaturonase of C. parasitica did not hydrolyze calcium polypectate except in the presence of oxalate ions.更多还原