【摘要】 虫花菌(Isaria farinosa(Dicks)Fr.)发酵液经超滤浓缩、乙醇沉淀、弱酸性阳离子交换树脂和弱碱性阴离子交换树脂去杂蛋白后,又经Sephadex G-150纯化得到PG。PG经醋酸纤维膜电泳、聚丙烯酰胺凝胶电泳和Sepharose 4B柱层析,证明是单一均匀的糖蛋白。PG的分子量为11.1万。PG的糖含量为92.35％、蛋白含量为7.61％。PG用气相色谱、红外光谱分析表明含有D-甘露糖、D-半乳糖,其摩尔比是5.93:1。推测PG主要含α-型糖苷键。PG对小鼠实体瘤S-180有一定的抑制作用,抑瘤率为22.2％。更多还原

【Abstract】 An extracellular proteoglycan PG was isolated from the culture filtrate of Isaria farinosa (Dicks) Fr. The crude preparation was obtained by ultrafiltration, three volumes of ethanol precipitation and acetone drying, and purified by resin 122#(H), Amberlite resin IR-45(OH) and Sephadex G-150 chromatography.PH was homogenous, as determinedly gel filtration on Sepharose 4B, acetic fibre paper electrophoresis and polyacrylamide gel electrophoresis. The average molecular weight of PG was 111000. PG is mainly consisted of polysaccharides (92.35%) together with small proportion of proteins (7.61%). PG was investigated by G.L.C. and I. R. spectroscopy. The results indicated that PG was composed of D-mannose and D-galactose in the molar ratio of 5.93:1 and linked mainly by a -glycoside bond. PG shows inhibitory activity against implanted Sarcoma 180 in mice. The percentage of inhibition is 22.2%.更多还原