【摘要】 本研究调查了日本血吸虫感染的ＢＡＬＢ／Ｃ小鼠脾细胞的ＩＦＮ－γ和ＩＬ－４ｍＲＮＡ转录。于感染后０，３，５，８，１０和１２周摘取小鼠脾脏，将脾细胞置于含ＳＥＡ或ＣｏｎＡ的培养基中培养，然后提取脾细胞总ＲＮＡ，通过逆转录后ｃＤＮＡ的扩增，分析ＩＦＮ－γ和ＩＬ－４的ｍＲＮＡ水平。ＲＮＡ模板同时进行β－ａｃｔｉｎ的逆转录和扩增，扩增产物被用作ＩＦＮ－γ和ＩＬ－４扩增的标准对照。在ＳＥＡ或ＣｏｎＡ诱导的未感染或感染３周小鼠脾细胞未检测到ＩＦＮ－γ和ＩＬ－４ｍＲＮＡ逆转录ＰＣＲ产物，感染５周和８周的小鼠脾细胞则可以观察到ＩＦＮ－γ和ＩＬ－４ｍＲＮＡ的表达。与感染５周时相比，ＳＥＡ刺激的感染８周小鼠脾细胞的ＩＬ－４ｍＲＮＡ转录显著增强。然而，在感染１０周和１２周小鼠，无论ＣｏｎＡ或ＳＥＡ都不能诱导ＩＦＮ－γ或ＩＬ－４ｍＲＮＡ转录，显示这两种淋巴因子表达受到抑制。结果表明，ＳＥＡ诱导的日本血吸虫感染小鼠脾细胞ＩＦＮ－γ和ＩＬ－４基因转录存在着调节，这种调节可能与感染从急性期到慢性期的对血吸虫虫卵抗原的全身性免疫反应包括对肉芽肿形成的调控有关。更多还原

【Abstract】 The transcription of mRNAs for IFN γ and IL 4 was investigated in S. japonicum infected BALB/c mice. Spleens were removed at 0,3,5,8,10 and 12 weeks after infection and the spleen cells were incubated in the presence of SEA or ConA. The extracted spleen cell RNA was analyzed for IFN γ and IL 4 mRNAs by PCR cDNA amplification after reverse transcription. The simultaneously reverse transcribed and PCR amplified β actin mRNA product was used as the control for IFN γ and IL 4 normalization. No detectable IFN γ and IL 4 mRNA RT PCR products was found in SEA or ConA treated spleen cells from uninfected, or 3 week infected mice, whereas IFN γ and IL 4 mRNAs were found to be prominently expressed in 5 and 8 week infected mice, and an appreciable enhanced expression of IL 4 mRNA was observed in SEA stimulated spleen cells at 8 week infection than at 5 week. However, neither ConA nor SEA could induce IFN γ or IL 4 mRNA transcription in 10 and 12 week infected mice indicating the modulated expression of mRNAs of these two lymphokines. These investigations demonstrated that the regulation of SEA induced IFN γ and IL 4 gene expression exists in S.japonicum infected mice and this regulation might be involved in the regulation of SEA related systemic immune responses including the modulation of granuloma formation occurred as the infection proceeds from acute to chronic stage.更多还原