【摘要】 为探讨血浆中５－羟色胺和儿茶酚胺的测定方法，采用改良的Ｋｅａｔｉｎｇ方法即高效液相色谱电化学检测技术测定兔血浆中５－羟色胺和儿茶酚胺，其方法灵敏、简单，以ＹＷＧ－Ｃ１８Ｈ３７为固定相，用反相色谱测定５－羟色胺，回收率达９０％以上，变异系数为３．３％。用反相离子对色谱分析儿茶酚胺，样本经氧化铝吸附后，用０．０５ｍｏｌ／ＬＨ３ＰＯ４洗脱效果更佳。儿茶酚胺回收率分别为７９．９％（去甲肾上腺素），９５．５％（肾上腺素）和８５．５％（多巴胺）。更多还原

【Abstract】 The concentrations of serotonin and catecholamine in plasma of rabbits were measured using a improved Keating’s method:high-performance liquid chromatography with electrochemical detection technique. This method is more specific,sensitive and simple than other methods.The concentration of serotonin was measured by reversed-phase high-performance liquid chromatographic method,in which YWG ODS was used as solid-phase column Φ4×250mm(10μm).The recovery of the serotonin was 93% and the coefficient of variation was 3.3%. The concentration of catecholamine was measured using a revered-phase ion-pair high-performance liquid chromatography technique.It is better to be adsorbed by aluminium oxide than washed by 0.05mol/L H 3PO 4. The recovery of catecholamine was 79.9%(NE) 95.5%(E) and 85.5%(DA)respectively.更多还原