【摘要】 本文报道了以甲苯胺兰键合修饰浸蜡石墨电极为基体电极,将葡萄糖脱氢酶（GDH）,烟酰胺腺嘌呤二核苷酸（NAD⁺）用戊二醛一起固定在蚕丝蛋白膜上,构成葡萄糖脱氢酶传感器。在pH=7.4的NaOH—KH₂PO₄介质中,该传感器与葡萄糖浓度在2.0×10^-5—6.4×10^-4mol/L范围内有良好的线性关系。响应叶间为20s。本文讨论了影响传感器响应电流的各种因素和测试葡萄糖的最佳条件。用此传感器测定了人血清中葡萄糖含量。与酶法结果一致。更多还原

【Abstract】 An amperometric glucose dehydrogenase biosensor was reported. It is made up of Toluidine Blue modified graphite electrode of immerse wax and enzyme film by glucose dehydrogenase, nicotinamide adenine dinucleotide immobilized on silk. In pH)=7.4 NaOH-KH2PO4 medium, the biosensor has a linear response in. the range of 2.0×10-3 - 6.4 × 10-3 mol/L for concentration of glucose The response time is about 20s. The factors affected the response current of sensor and the optimical conditions of determination of glucose have been discussed in details. The sensor has been used odetect the concentration (?) and the result conform to those obtained by enzyine analytical method.更多还原