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离子注入法导入外源DNA引起陆地棉性状变异的研究
STUDY ON CHARACTER VARIATION INDUCED BY INTRODUCING EXOGENOUS DNA INTO UPLAND COTTON WITH ION IMPLANTATION
【摘要】 通过30keV不同剂量的Ar+注入和DNA溶液浸滴法将比克氏棉和红麻DNA导入泗棉2号。结果表明:离子注入可显著地促进外源DNA导入受体,增加受体后代性状的变异频率和变异类型,而且多数变异可迅速趋于稳定。在供试剂量中,以2×1015Ar+/cm2介导外源DNA引起受体性状变异的频率最高,达16.2%。经选育已获得了一批抗枯萎病、早熟、种子少腺体和优质等各具特色的陆地棉新种质系。
【Abstract】 The exogenous DNAs of G.bickll P. and H.Cannabinus were introduced into the upland cotton Si 2 by Ar +implantation and DNA solution trickling method. The results showed that the exogenous DNA introduction was promoted significantly and the types and frequencies of character variation in progeny were increased by Ar + implantation. Furthermore, most of the variation tend to be stable. Among the Ar +implantation doses tested, 2×10 15 Ar +/cm 2 was the best for introducing exogenous DNA and inducing character variation, the variation rate reached to 16 2%. Some new lines with character of resistance to wilt disease,early maturity, few gland in seed and fine fiber quality have been obtained.
【Key words】 Ion implantation; exogenous DNA introduction; upland cotton; variation;
- 【文献出处】 核农学报 , 编辑部邮箱 ,1996年03期
- 【分类号】S124.1:S562
- 【被引频次】76
- 【下载频次】744