【摘要】 用紫外吸收光谱，荧光光谱，荧光淬灭等手段研究一系列钌配合物ＲｕＬ２ｐｄｐｈｅｎ２＋与鱼精ＤＮＡ的作用，这里，Ｌ分别为２，２′－联吡啶，邻菲咯啉，２，９－二甲基－邻菲咯啉和５－硝基－邻菲咯啉，ｐｄｐｈｅｎ为吡啶并［３，２－ｆ］［１，７］邻菲咯啉。所有这些配合物与鱼精ＤＮＡ作用时，总是表现出吸收光谱峰ＭＬＣＴ的减色效应，对于配合物Ｒｕ（ｂｉｐｙ）２ｐｄｐｈｅｎ２＋和Ｒｕ（ｐｈｅｎ）２ｐｄｐｈｅｎ２＋，还表现出荧光强度增加。所有的结果表明配合物与鱼精ＤＮＡ的作用仍为插入作用，但配合物与鱼精ＤＮＡ的作用强度弱于与小牛胸腺ＤＮＡ作用的程度更多还原

【Abstract】 The binding of RuL 2pdphen 2+ to herring sperm DNA was investigated using absorption,fluorescence spectroscopies and fluorescence quenching, where pdphen is pyrido phenanthroine, L are 2, 2′-bipyridine, phenanthroine,2,9-dimethyl-phenanthroline and 5-nitro-phenanthroline respectively. All complexes show absorption hypochromicity associated with binding to herring sperm DNA.Ru(bipy) 2pdphen 2+ and Ru(phen) 2pdphen 2+ also show fluorescence intensities increase.Interaction of complexes with herring sperm DNA is weaker than that of with calf thymus DNA.更多还原