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磁场对固定化乳酸脱氨酶催化活性的影响
Effect of Magnetic Field on Activity of Immobilized LDH
【摘要】 采用流通池光纤荧光光度法,对固定化乳酸脱氢酶(LDH)在静磁场作用下的催化活性进行了探讨.发现在0.084~0.230T磁场作用下LDH的催化活性明显降低,其降低值随磁场强度增强而增大,且与磁场作用的时间和pH值有关,而在25~40℃范围内,温度则无显著影响。
【Abstract】 The catalytic activity of immobilized LDH in static magnetic field has been investigated using fiber optical fluorimetry with flow cell.Remarkable decrease of the activity of LDH is observed in a magnetic intensity range of 0.084 to 0.230 T.Asthe magnetic intensity is increasing until up to 0.230 T.the activity of LDH is decreasing until by-17%.This effect is related to magnetization time and pH of subtrate solution, while temperature has no effect in the range of 25-40℃
【关键词】 磁场;
乳酸脱氢酶;
光纤荧光光度法;
催化活性;
【Key words】 magnetic field; lactate dehydrogenase; fiber optic fluorimerty; catalytic activity.;
【Key words】 magnetic field; lactate dehydrogenase; fiber optic fluorimerty; catalytic activity.;
【基金】 国家自然科学基金
- 【文献出处】 湖南大学学报(自然科学版) ,JOURNAL OF HUNAN UNIVERSITY(NATURNAL SCIENCES) , 编辑部邮箱 ,1996年05期
- 【分类号】Q55
- 【被引频次】4
- 【下载频次】45