【摘要】 本研究分析了卵巢不同离体时间（＜３ｈ、４～５ｈ、＞６ｈ），不同培养时间（１８～２２ｈ、２３～２４ｈ、＞３０ｈ），不同成熟培养液（添加ＦＳＨ、ＨＣＧ、ＦＣＳ）以及不同培养温度（３４℃、３６℃、３８．５℃）对卵母细胞体外成熟的影响。结果表明，小于３小时离体时间成熟率、受精率（７８．８３％、５８．０％）高于其它组；培养时间大于３０小时组成熟率高于其它组（９３．８１％），但受精率低于其它组（２３．０％）；添加ＨＣＧ与ＦＣＳ组成熟率、受精率（８９．４％、７８．９５％）大于ＦＳＨ组；培养温度以３８．５℃为佳，成熟率与受精率（８７．２％、７３．６％）皆高于另二组，差异显著。更多还原

【Abstract】 Analyze the influence of different overy transportation time (from slaugter site to lab ) (less than 3 hours, 4-5 hours, more than 6 hours), different culture time (18-22 hours, 23-24 hours, 30 hours ) and different culture temperature (34℃, 36℃, 38.5℃) on in vitro maturation of bovine oocytes. The results show that the maturation and fertilization rate of less than 3 hours groups (78.83%, 58.0%) are highest. The oocyte cultured nore than 30h was highest in maturation rate (93.81%), but the fertilization rate is lower than other groups. The group adding HCG has higher maturation and fertilization rate than that of FSH group. The best culture temperature is 38.5℃, the two rates are highest among the three groups with significent difference.更多还原