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两种假单孢菌中二氯酚降解酶活性及其定域研究
STUDY ON ENZYME ACTIVITY AND DISTRIBUTION IN TWO STRAINS OF BACTERIA BY DICHLOROPHENOL DEGRADATION
【摘要】 对两种有降解二氯酚能力的假单孢菌Pseudomonassp.DCP-1和Pseudomonassp,DCP-2中二氯酚降解酶活性及定域进行了测定,结果表明该酶的活力与菌种、培养时间等因素有关,与诱导物的关系不显著;酶主要定域在膜周及膜内;酶的活力在两种菌中的水平相当;酶的比活力在CDP-1的膜周处表现出相当高的水平。
【Abstract】 Activity and distribution of enzyme produced by two strains of bacteria (Psedomonas sp.DCP-1 and Psedomonas sp. DCP-2) , which can utilize dichlorophenol, have been measured.The results shown that enzyme activity is effected by bacteria and culture time, and not by inducer; the distribution of enzyme produced by the two strains of bacteria is mainly in piroplasmic and intercellular; the specific activity in piroplasmic of Pseudomonas, sp. DCP-1 is higher than that in DCP-2.
【关键词】 二氯酚;
降解酶;
比活力;
定域;
假单胞菌CDP-1;
假单胞菌DCP-2;
【Key words】 dichlorophenol; degrading-enzyme; specific activity; distribution; Pseudomonas sp. DCP-1; Pseudomonas sp. DCP-2.;
【Key words】 dichlorophenol; degrading-enzyme; specific activity; distribution; Pseudomonas sp. DCP-1; Pseudomonas sp. DCP-2.;
【基金】 高等学校博士学科点专项基金
- 【文献出处】 环境科学学报 ,ACTA SCIENTIAE CIRCUMSTANTIAE , 编辑部邮箱 ,1996年02期
- 【分类号】X131
- 【被引频次】61
- 【下载频次】205