【摘要】 由胆绿素经钠硼氚还原生成氚标记胆红素，而胆绿素是由胆红素经ＤＤＱ脱氢氧化形成，钠硼氚是在镍催化及高温下由销硼氢与氚气交换得到。氚标记胆红素比活度为３０６ＴＢｑ／ｍｏｌ，放化纯度经高效液相色谱及纸层析鉴定均大于９５％，用氚标记胆红素进行开放血脑屏障的动物实验获得了满意的结果。更多还原

【Abstract】 ynthesis of 3H-labelled bilirubin is described.3H-bilirubin is prepared by the reduction of biliverdin using sodium boro-[3H]-hydride in methanol solvent.But biliverdin is synthesized through dehydrogenation of bilirubin with 2, 3-dichloro-5,6-dicyanobenzoquinone(DDQ)in dimethyl sulphoxide and sodium boro-[3H]-hydride is produced by exchange of sodium boro-hydride with tritium gas using nickel catalyst at high temperature.The specific activity of obtained 3H-bilirubin is 306GBq/mmol,while the radiochemical purity is over 95% by HPLC and paper chromatography.The permeatied profile of 3H-labelled bilirubin in rat brain has been satisfyingly obtained in animal experiments.更多还原