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慈菇蛋白酶抑制剂对亚洲玉米螟γ-谷氨酰转移酶和氨基酸吸收的影响
THE EFFECT OF ARROWHEAD PROTEINASE INHIBITOR ON γ-GLUTAMYL TRANSPTIDASE(γ-GTP)OF ASIAN CORN BORER,OSTRINIAFURNACALIS AND ON ITS ABSORPTION OF AMINO ACIDS
【摘要】 正常玉米螟血淋巴内游离氮基酸总量为22.32mg/ml。当幼虫短时间(四龄未至五龄第二天)和长时间(三龄初至五龄第二天)取食慈菇蛋白酶抑制剂(AHI)后,游离氮基酸总量分别为对照的73.9%和33.3%,大多数氮基酸含量明显低于对照;血液蛋白质浓度下降20-52%;幼虫体内γ—谷氨酰转肽酶(γ—GTP)活力也明显下降,但体外不被AHI抑制。这些结果表明:AHI能有效地抑制幼虫蛋白水解酶,并间接地影响其他酶和蛋白质的生物合成,最终导致幼虫生长不良。
【Abstract】 The total amount of amino acid in the haemolymph of normal larvae of A sian cornborer,Ostrinia furnacalis was 22.32 mg/ml.When the larvae acutely and chronically were in- gested with artificial diets containing AHI,the amount of free amino acids in haemolymph was73.9%and 33.3%of that of control respectively,and the content of overwhelming majority ofall aniino acids to two treatments was lower than that in control,especially in chronic treatment.AHI decreased in vivo the level of γ-glutamyl transpeptidase(γ-GTP),but the in vitro effectof AHl on γ-GTP activities in the larval preparations was not significantly different compared tothat of control.These results indicate that AHI effectiyely affects the larval synthesising otherproteins and enzymes,resulting in insects abnormal growth.
【Key words】 arrowhead proteinase inhibitor; Ostrinia furnacalis; γ-glutamyl transpepti- dase; Amino acid;
- 【文献出处】 华东昆虫学报 ,ENTOMOLOGICAL JOURNAL OF EAST CHINA , 编辑部邮箱 ,1996年02期
- 【分类号】Q965
- 【下载频次】48