【摘要】 本实验探讨了单克隆抗体Ａｎｔｉ－ＬＡ１８对人外周血单个核细胞（ＰＢＭＣ）的影响。ＬＡ１８细胞株培养７２ｈｒ后收集合Ａｎｔｉ－ＬＡ１８的上清；取健康人静脉血分离单个核细胞；用３Ｈ－ＴｄＲ掺入法测定培养于含１０％Ａｎｔｉ－ＬＡ１８上清的ＰＢＭＣ，发现其增殖高峰在第３～６天。培养后第３天，Ａｎｔｉ－ＬＡ１８作用组出现淋巴母细胞、体积较大的单核细胞以及细胞岛样结构，电镜下进一步证实了以上结果。酶细胞化学显示Ａｎｔｉ－ＬＡ１８组单核细胞ＡＮＡＥ活性较对照组明显增强，而ＰＯＸ活性减弱。结合以往的研究，结论如下：Ａｎｔｉ－ＬＡ１８是一种人特异性单克隆抗体，它能直接引起ＰＢＭＣ的增殖，并能诱导淋巴母细胞的出现和促进单核细胞分化为巨噬细胞。更多还原

【Abstract】 Abstract This study aims at defining the effect of Anti-LA 18 on human PBMC. Anti-LA18 supernate (SN) was’ collected from LA18 cell line afterbeing cultured 72 hrs later. PBMCswere isolated from normal healthy donor,s vein blood. PBMCs were cultured in 10% AntiLA18 SN and harvested on the 3rd day, then prepared for cytochemistry and electron microscope (EM) detemination. The results showed that the cell,s proliferation peak was on day 3-- 6. The lymphoblasts and larger monocytes appeared Oh the 3rd day after culturing in Antic- LA18 SN. This result was further confirmed by electron misroscope. Monocytes showedANAE activity and the POX.activity decreased obviously in Anti--LA18 SN group comparedwith control group. According to these findings-and our past studies, we conclude that theAnti--LA18 SN is a specific human monoclonal antibody. It can directly stimulate the proliferation and differentiation’ of PBMCs. Also Anti-AIA18 SN can induce the presence of lymphoblast and promote differentiation of monocyte into macrophage,Keywrods: monoclonal antibody Anti-LA18 SN; humane periperal bloodmononuclear cell (PBMC)更多还原