【摘要】 毛细管电泳的微机化安培法检测胡深，李培标，程介克（武汉大学化学系，武汉，４３００７２）关键词毛细管电泳；微电极；安培法检测；微型计算机安培法电化学检测器用于毛细管电泳有独特的优点，如线性范围宽，选择性好，可以进行生物微环境电活性物质的分离检测［１，２...更多还原

【Abstract】 A computerized end-column amperometric detector for capillary zone electrophoresis is constructed. The microelectrode is positioned at the end of 25 μm i. d. capillary by micromanipulator directly.The electrode potential is controlled and the signal is sampled by the microcomputer.Application of the system to the separation and detection of NE, IP and CAT is demonstrated. This system is also applied to investigate the field-amplified injection in capillary electrophoresis with amperometric detection and the concentration limits of 0.45～0.65nmol/L for DA, NE, E and IP are achieved.更多还原