【摘要】 用再生丝素把过氧化物酶（POD）固定在玻碳电极表面，以N-甲基吩嗪硫酸盐（PMS）为电子传递体，制成过氧化氢传感器。因该酶电极对过氧化氢（H2O2）有良好的催化响应，PMS还原电流的增值与过氧化氢的浓度在10－6～10－3mol／L范围内有良好的线性关系，酶电极稳定性好，灵敏度高，对过氧化氢的响应时间小于10s，其有效寿命可达二个月以上。用扫描电镜和红外光谱观察和分析了POD和再生丝素共混膜的表面形态和结构。更多还原

【Abstract】 A H2O2 sensor is prepared by immobilizing peroxidase with regenerated silk fi broin on the surface of a glassy-carbon electrode and employing phenazine methosulphate (PMS) as an electron shuttle. This sensor possesses high sensitivity to H2O2 and good stabil ity. There is a linearity between the increment of reduction current of PMS and H2O2 concen tration within the range of 10-6~ 10-3 mol/L with the response tirne of less than 10 s and service time of more than two months. The structure of the blend membranes of peroxidase and regenerated silk fibroin are investigated with IR and scanning electron microscopy.更多还原