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干旱胁迫对番茄活性氧代谢的影响
Effects of Drought Stress on Activated Oxygen Metabolism in Tomato
【摘要】 干旱胁迫导致番茄叶片RWC、Chl和可溶性蛋白质含量下降,质膜透性和ATPase活性提高.短期干旱胁迫可提高叶片SOD和CAT活性.4d以内的干旱胁迫后复水,RWC、Chl和可溶性蛋白质含量升高,清除活性氧保护酶SOD、POD和CAT的活性显著提高,抗氧化物质AsA和GSH的水平得到恢复,MDA得到清除,自动氧化速率降低,重新建立了活性氧产生与清除的平衡系统,ATPase活性降低,膜系统的损伤得到修复,透性降低,恢复了原有正常的生理代谢机能.长期干旱胁迫导致叶片清除活性氧的平衡体系受到破坏,SOD、POD和CAT活性以及AsA和GSH的含量下降,膜脂过氧化加剧,自动氧化速率提高,MDA积累,影响番茄正常代谢,从而加速衰老进程
【Abstract】 Relative water content (RWC) and the content of chlorophll (Chl) and soluble protein were reduced, and the rate of membrane permeability and ATPase activity increased in leaves of tomato, caused by drought stress. It was found that the activities of superoxide dismutase (SOD) and catalase (CAT) were increased under slight stress of drought. The results indicated that the contents of Chl and soluble protein, RWC and activities of 3 protective enzymes (SOD, CAT and POD) were increased significantly, the contents of AsA and GSH were increased too when watering was recommenced after less than 4 day drought stress, MDA was scavenged and the rate of tissue autoxidation was decreased, i.e. the balance system of activated oxygen being produced and scavenged was re established, the rate of permeability and ATPase activity was reduced, the damage to membrane system was repaired, and normal physiological metabolism was recovered. And the activities of SOD, CAT and peroxidase (POD) and the contents of ascorbic acid (AsA) and glutathione (GSH) were decreased, lipid peroxidation increased, the rate of tissue autoxidation, an indicator of the protective mechanism of tissue, increased greatly and the content of malonaldehyde (MDA) accumulated under severe stress of drought, i.e. the ability to scavenge activated oxygen was decreased significantly, which affected the normal metabolism and accelerated leaf senescence.
【Key words】 tomato; drought; activated oxygen metabolism; lipid peroxidation; senescence;
- 【文献出处】 福建农业大学学报 ,JOURNAL OF FUJIAN AGRICULTURAL UNIVERSITY , 编辑部邮箱 ,1996年03期
- 【分类号】S630.1
- 【被引频次】95
- 【下载频次】485