【摘要】 本文提出了荧光法测定强力霉素（DC）的新方法。强力霉素碱性降解后与铝离子形成强荧光的络合物,并据此进行荧光测定,其荧光强度在4.0×10^-8～7.0×10^-6mol/L 范围内与强力霉素浓度成正比,共存的其它四环素类抗菌素不干扰强力霉素的测定,测定的检测限为3.0×10^-8mol/L。此法用于血清和尿液中强力霉素的测定,回收率分别为93～97%和91%～99%。更多还原

【Abstract】 A new fluorimetric method for the determination of trace doxycycline is proposed. The Alkaline degradation product of doxycycline（isodoxycycline）reacts rapidly with AI³⁺ and produces strong fluorescent chelate which is detected by fluorimetry. The fluorescence intensity of the chelate is proportional to the concentration of doxycyctine over the range of 4.0×10^-8～7.0×10^-6mol/L.Coexisting methacyctine and other tetracyclines do not inter- fere in the determination of doxycycline.The detection limits is 3.0×10^-8mol/L.This method has been used for the determination of doxycycline in serum and urine with 93%～ 97% and 91%～99% recoveries,respectively.更多还原