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金耳水溶性多糖的部分化学性质及抑瘤试验
Preliminary study on chemical properties of a soluble polysaccharide from the mushnoom Tremella aurantia, and its oncosuppressive activity
【摘要】 金耳(Tremellaaurantia)子实体经热水抽提,反复经Sevag法脱蛋白,获得金耳多糖.Sepharose4B分级分离呈现双峰(A和B).其峰A的相对分于质量介于105~106之间.峰A组分经醋酸纤维薄膜电泳鉴定,呈现单一谱带,为一纯多糖.利用硅胶G薄板层析技术,确定峰A组分主要由葡萄糖、甘露糖、半乳糖组成.利用人肺腺癌裸小鼠模型LAX-83对金耳多糖全组分进行抑瘤试验,结果表明有明显抑瘤疗效.
【Abstract】 A soluble polysaccharide was extracted by hot-water from the mushnoom Tremella aurantia. and other proteins were removed by the Sevag procedure. The polysaccharide showed two peaks(A and B) in Sepharose 4B chromatography. peak A was of a Mr. 105-106 and its major components were glucose .galactose and manose. A marked oncosuppressive activity was demonstrated by feeding and injecting pert tonealy the polysaccharide to nude mice carrying tumor(LAX-83).
- 【文献出处】 复旦学报(自然科学版) , 编辑部邮箱 ,1996年06期
- 【分类号】R282.71
- 【被引频次】13
- 【下载频次】141