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应用单克隆抗体检测血吸虫病人血清特异性循环免疫复合物的初步研究

Preliminary Stumes on the Detection of Circulating Immune Complex in Serum from Patients with Schistosomiasis by Monoclonal Antibodies

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【作者】 郑志国楼涤郁俊豪蒋健敏汤益屠乐鸣

【Author】 Zheng Zhiguo; Lou Di; Yu Junhao;et al(Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, Hangzhou 310013)

【机构】 浙江省医学科学院寄生虫病研究所

【摘要】 抗血吸虫McAb应用于ELISA(McAb一ELISA)检测血吸虫病人血清中抗原特异性CIC,并与其提取的CIC沉淀物为对照,二者结果完全相同。另与“抗原非特异性”CIC检测法(PEG法)作对照。慢件血吸虫病患者67例,McAb一ELISA有46例呈阳性反应(68.7%),21例呈阴性反应(31.3%),对肺吸虫病患者59例、健康人23例全部呈阴性反应;而PEG法,肺吸虫病患者全部呈假阳性反应,健康人6例呈假阳性反应(26%)。初步结果表明,McAb一ELISA检测血清CIC具有较高的抗原特性。

【Abstract】 Specific CIC in sera from patients with schistosomiasis japonica were demonstrated by usingMcAb( Ⅲ D10)- ELISA,and its extracted CIC as contrast.Both results were completely same. There was com- parison between this McAb- ELISA and PEG test.67 sera from patients of chronic schistosomiasis were de- tected by McAb-ELISA. 46 sera were positive(68.7%)and 21 sera were negative(31.3%).59 sera of pa-tients of paragonimiasis and 23 sera of normal persons were all negative. But PEG test all of patients ofparagonimiasis and 6 sera(26%)of normal persons were false positive. So the preliminary results of thisstudy showed that CIC in serum detected by this method has high antigenic specificity.

  • 【文献出处】 地方病通报 ,ENDEMIC DISEASES BULLETIN (CHINA) , 编辑部邮箱 ,1996年02期
  • 【分类号】R532.21
  • 【被引频次】1
  • 【下载频次】9
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