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小麦未熟胚离体培养的研究──再生植株后代花粉粒育性观察
STUDIES ON IMMATURE EMBRYO CULTURE IN VITRO IN WHEAT(TRITICUM AESTIVUM L.)──Pollen fertility in Progeny of regenerated plant
【摘要】 试验以IE8515-28(77(2)春品种的胚培后代)14个早熟株系的IE5代植株为材料,观察植株后代花粉粒育性.结果表明.小麦未熟胚离体培养后所产生的后代,结实均表现正常,但在成熟花粉粒育性观察中发现空瘪、小型以及多萌发孔等畸形花粉粒.而且不同株系各种异常花粉粒所占比例不同.说明胚培无性系后代遗传基础受到深刻影响,包括小孢子形成和发育的全过程.
【Abstract】 The IE5 generation of 14 lines early in maturity derived from embryo culture progeny IE 8515-28 of cultivar 77(2)-Spring were used as the materials for pollen fertility study. The results showed that the performance of seed-setting in progenics from immature embryo culture in vitro was normal but various types of abnormal pollen were found, including the empties,the smallest and multi-germination poles in different percentages.It indicated that the genetic base of the somaclonal progeny from embryo culture in vitro was significantly influenced and involved the formation of small spores.
【Key words】 wheat; Triticum aestivum L.pollen; fertility; immature mbryo culture; somaclone;
- 【文献出处】 东北农业大学学报 ,JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY , 编辑部邮箱 ,1996年02期
- 【分类号】S512.103.5
- 【被引频次】2
- 【下载频次】57