【摘要】 用正交设计法对影响桑子叶不定芽形成的诸因素进行了比较研究。结果得出：（１）诱导培养基采用ＭＳ无机盐加即维生素、６－苄氨基嘌呤３．０ｍｇ／Ｌ、吲跺乙酸０．３ｍｇ／Ｌ、果糖或葡萄糖３０ｇ／Ｌ、水解乳蛋白５００ｍｇ／Ｌ和琼脂粉６ｇ／Ｌ最为适合，不定芽诱导率达到８０％以上；（２）子叶初期培养中，光照以５００～１０００ｌｘ较为适宜；（３）预培养４～６ｄ的桑种子，其子叶不定芽诱导率较其余子叶为高。不定芽经继代培养后能在生根培养基中形成完整根系，移植到土壤后可成苗。更多还原

【Abstract】 The factors influencing the formation of adventitious buds were studied by means oforthogonal design.The results showed that,(1)A medium containing MS mineral salts,8p vitamins,3.0mg/l of 6-benzylaminopurine,0. 3mg/l of indole-3-acetic acid,30g/l offructose or glucose,500mg/l oflactalbumin hydrolysate,solidified with 6g/l of agarpowder was most favorable for inducing adventitous buds from mulberry cotyledons.With this medium,the induction rate reached more thn 80%,(2)During the initial cul-ture of the cotyledons,illumination at an intensity of 500-1000lx is desirable,(3)Cotyle-dons isolated from mulberry seeds that had undergone preculture mediurn and subse-quently rooted well in rooting medium.更多还原