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抗生素对桑树外植体生长与分化的影响

EFFECTS OF ANTIBIOTIC ON GROWTH AND DIFFERENTIATION OF MULBERRY EXPLANTS

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【作者】 王勇陈爱玉夏志松李雪明倪国孚

【Author】 Wang Yong;Chen Aiyu;Xia Zhisong;Li Xueming;Ni Guofu(Sericultrual Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang 212018,China)

【机构】 中国农业科学院蚕业研究所

【摘要】 试验比较了不同种类抗生素在不同浓度下对桑子叶、不定芽和新梢生长与分化的影响,结果显示:(1)培养基中卡那霉素浓度高于20mg/L时.桑子叶不能形成不定芽;羧苄青霉素或头孢霉累大于400mg/L时,子叶不定芽诱导率明显下降。(2)卡那霉素大于30mg/L,羧苄青霉素或头孢霉累大于400mg/L,不定芽抽茎受到明显抑制。(3)卡那霉素大于10mg/L,羧苄青霉素或头孢霉素大于100mg/L,桑树新梢难以形成根系。

【Abstract】 Effects of diHerent antibiotics at various concentrations on the growth and differen-tiation of mulberry cotyledons,adventitious buds and shoots were studied.The resultsshowed that :(1)Mulberry cotyledons could not form adventitious bud while concentra-tion of kanamycin in the medium was higher than 20 mg/L:the induction rate of adven-titious buds was obviously lowered while the concentration of carbenicillin or cefazolinsodium was over 400 mg/L,(2)Elongation of adventitious buds was remarkably inhibit-ed while the concentration of kanamvcin and carbenicilin or cefazolin sodium was higherthan 30 mg/L and 400 mg/L respectively.(3) Mulberry shoots could not form healthyroots while kanamycin and carbenicillin or cefazolin sodium was at 10 mg/L and 100 mg/L,respectively.

【关键词】 桑树 外植体生长抗生素
【Key words】 Mulberry Explant Growth Antibiotic
【基金】 国家自然科学基金
  • 【文献出处】 蚕业科学 ,ACTA SERICOLOGICA SINICA , 编辑部邮箱 ,1996年02期
  • 【分类号】S888.2
  • 【被引频次】41
  • 【下载频次】110
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