【摘要】 目的：探索人重组白细胞介素－８（ｒｈＩＬ－８）的分离纯化的路线。方法：用Ｓｅｐｈａｃｒｙ１－Ｓ－２００凝胶过滤和Ｑｓｅｐｈａｒｏｓｅｈｉｇｈｐｅｒｆｏｒｍａｎｃｅ，Ｑｓｅｐｈａｒｏｓｅｂｉｇｈｅａｄｘ两步连续阴离子交换层析纯化。结果：两种方法纯化的ｒｈＩＬ－８纯度分别达９５．６％、９８．６％。氨基酸组份分析推算值基本与理论值相符。纯化的ｒｈＩＬ－８对小鼠和人中性粒细胞具有明显的体内体外趋化作用。结论：本法为ｒｈｌＬ－８提供了快速高效的纯化路线。更多还原

【Abstract】 Objective:To study the purification and identification of recombinant IL-8.Methods:Two protocols were performed and compared.The first one purified rhlL-8 via passaging over Sephacryl-S-200 gel-filtration chromatography.The second one by two step ion-exchange chromatography.Results:The purified rhIL-8 were 95.6%and 98.6%homogeneity as analysis with SDS-PAGE,Respectively. and exhibited chemotaxis for neurophils in vitro and in vivo. Conclusion: The results provide rapidly and efficiently protocols for the purificatlon and identification of rhlL-8.更多还原