节点文献

黄腐酸和超氧阴离子对软骨细胞内钙离子浓度的影响

THE EFFECT OF FULIC ACID AND SUPEROXID ANION ON INTERCELLULAR CALCIUM ION CONCENTRATION

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 许善锦陈瑞新楼晓燕王夔

【Author】 XU Shanjin; CHEN Ruixin; LOU Xiaoyan; WANG Kui(Department of Inorganic Chemistry. Beijing Medical University 1 00083)

【机构】 北京医科大学无机化学教研室

【摘要】 目的:为探讨病区黄腐酸(FA)和活性氧自由基可能引起大骨节病,我们测定了在FA和超氧自由基()作用下,软骨细胞内钙离子浓度([Ca ̄(2+)]i)随时间的变化。方法:应用萤光指示剂Fura-2/AM测定细胞内钙离子浓度并用下式计算:[Ca ̄(2+)]i=Kd(F-F_(min))/(F_(max)-F)。结果:作用45min后,[Ca ̄(2+)]i从1.62×1O ̄(-7)mol/L升达1.18×10 ̄(-6)mol/L;FA作用4h后,[Ca ̄(2+)]i从3.78×10 ̄(-7)mol/L升达5.51×10 ̄(-7)mol/L。结论:说明FA与·有相似的促进[Ca ̄(2+)]i升高的作用,[Ca ̄(2+)]i的升高将造成软骨细胞损伤,而软骨坏死是大骨节病的重要病理特症之一。

【Abstract】 s Objective . The variation of intercellular calcium ion concentrations of chondrocytes([Ca ̄(2+)] )itreated by fulic acid(FA ) and superoxide anion()was measured.so that we can understand kashin-Beck’s Disease (KBD) induced by FA and oxygen free radical. Methods. [ Ca ̄(2+)]iwas measured by using fluorescent indicator. Fura-2/AM and calculated with the formula[Ca ̄(2+)]i=Kd (F-Fmin)/ (Fmax-F).Results . These results indicated that [Ca ̄(2+)]i increased from 1. 62 × 10 ̄(-7) mol/L to 1. 18 ×10 ̄(-6) mol/L when treated by· for 45 min and from 3. 78× 10 ̄(-7) mol/L to 5.51× 10 ̄(-7) mol/L when treated by FA for 4 h. Conclusious . Both FA and· made [Ca ̄(2+)]iincrease. which will cause dam- age to chondrocytes. The cartilage necrosis is one of the pathological Characteristics of KBD.

【基金】 国家八五科技攻关课题
  • 【文献出处】 北京医科大学学报 ,JOURNAL OF BEIJING MEDICAL UNIVERSITY , 编辑部邮箱 ,1996年02期
  • 【分类号】R684.105
  • 【被引频次】1
  • 【下载频次】61
节点文献中: 

本文链接的文献网络图示:

本文的引文网络