【摘要】 目的：为探讨病区黄腐酸（ＦＡ）和活性氧自由基可能引起大骨节病，我们测定了在ＦＡ和超氧自由基（）作用下，软骨细胞内钙离子浓度（［Ｃａ￣（２＋）］ｉ）随时间的变化。方法：应用萤光指示剂Ｆｕｒａ－２／ＡＭ测定细胞内钙离子浓度并用下式计算：［Ｃａ￣（２＋）］ｉ＝Ｋｄ（Ｆ－Ｆ＿（ｍｉｎ））／（Ｆ＿（ｍａｘ）－Ｆ）。结果：作用４５ｍｉｎ后，［Ｃａ￣（２＋）］ｉ从１．６２×１Ｏ￣（－７）ｍｏｌ／Ｌ升达１．１８×１０￣（－６）ｍｏｌ／Ｌ；ＦＡ作用４ｈ后，［Ｃａ￣（２＋）］ｉ从３．７８×１０￣（－７）ｍｏｌ／Ｌ升达５．５１×１０￣（－７）ｍｏｌ／Ｌ。结论：说明ＦＡ与·有相似的促进［Ｃａ￣（２＋）］ｉ升高的作用，［Ｃａ￣（２＋）］ｉ的升高将造成软骨细胞损伤，而软骨坏死是大骨节病的重要病理特症之一。更多还原

【Abstract】 s Objective . The variation of intercellular calcium ion concentrations of chondrocytes（[Ca￣（2+）] ）itreated by fulic acid（FA ） and superoxide anion（）was measured.so that we can understand kashin-Beck’s Disease （KBD） induced by FA and oxygen free radical. Methods. [ Ca￣（2+）]iwas measured by using fluorescent indicator. Fura-2/AM and calculated with the formula[Ca￣（2+）]i=Kd (F-F_min)/ (F_max-F).Results . These results indicated that [Ca￣（2+）]i increased from 1. 62 × 10￣（-7） mol/L to 1. 18 ×10￣（-6） mol/L when treated by· for 45 min and from 3. 78× 10￣（-7） mol/L to 5.51× 10￣（-7） mol/L when treated by FA for 4 h. Conclusious . Both FA and· made [Ca￣（2+）]iincrease. which will cause dam- age to chondrocytes. The cartilage necrosis is one of the pathological Characteristics of KBD.更多还原