【摘要】 Ｉ－６９杨插穗分别培养在含有不同浓度Ｃｅ（ＮＯ３）３的营养液中，经快速冷冻、冷冻干燥、无水塑料包埋及干刀薄切片法，用Ｘ－射线能谱微区分析测定Ｃｅ及其它无机离子在茎尖分生组织和叶的不同组织中细胞壁、细胞质、细胞核及叶绿体中的含量及分布。在１０ｐｐｍＣｅ（ＮＯ３）３处理下，茎尖中Ｃｅ的含量比对照组和１００ｐｐｍＣｅ（ＮＯ３）３处理组高出近１倍，叶的亚细胞中的Ｋ、Ｐ、Ｓ、Ｆｅ的含量也呈增加的趋势。特别是Ｍｇ的含量增加显著，在保卫细胞的细胞质中Ｍｇ的含量比对照组高出３．５倍，而在表皮细胞的细胞核和液泡中的含量则比对照组高出近１３倍。但在１００ｐｐｍＣｅ（ＮＯ３）３处理下，无机离子在叶中亚细胞微区间的含量则显著下降更多还原

【Abstract】 Cuttings of Populus deltoides were grown in solutions with different Ce(NO 3) 3 concentrations. After fast freezing, freeze-drying and water-free embedding in plastic, Ce and other ion concentrations were measured by X-ray microanalysis in cell wall, cytoplasm, nucleus and chloroplast of cells of stem tip meristem and leaves. In the stem tip, the Ce content of cutting exposed to 10 ppm Ce(NO 3) 3 was nearly twice as high as that in the control or cutting exposed to 100 ppm Ce(NO 3) 3. K, P, S and Fe content of leaves increased under 10 ppm Ce(NO 3) 3. In particular, Mg content in guard cell cytoplasm increased about 3.5 times than that of the control, and in the epidermis nucleus and vacuole, nearly 13 times that of the control. However, under 100 ppm Ce(NO 3) 3 treatment, the ion contents of most cellular compartments of leave were sharply decreased.更多还原