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虫生真菌的侵染与马尾松毛虫营养生理及生殖的关系的研究

Impact of Beauveria Bassiana Infection on Nutritional and Reproductive Physiology of Masson’s Pine Caterpillar,Dendrolimits Punctatus

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【作者】 李增智刘令峰刘玉珍

【Author】 Li Zengzhi;Liu Linfeng;Liu Yuzhen(Anhui Agricultural University,Hefei 230036)

【机构】 安徽农业大学森林利用学院

【摘要】 用高浓度(1×10 8分生孢子/ml)和低浓度(4×10 6)球孢白僵菌孢悬液处理4龄马尾松毛虫幼虫,在处理后至开始侵染死亡的15d内,高浓度处理减少松针损失44.4%,低浓度处理减少19.6%,消化率无显著差异,但食物利用率和转化率比对照低。饲养未死于侵染的残余幼虫至化蛹,其侵染率、化蛹率、羽化率、性比及蛹重有随着处理浓度的增加而降低的趋势。实验结束时检查受精卵数,高浓度、低浓度处理组和对照组分别为1283、4826和9604粒,实验种群的实际增长率分别为12.83、48.26和96.04。种群增长率因高浪度和低浓度处理而分别降低86.6%和49.8%,此外,还研究了饥饿对虫生真菌侵染4龄幼虫的影响。将幼虫分别喷以球孢白僵菌、金龟子绿僵菌和粉拟青霉后,饥饿处理1、2、3和4d后正常饲养,各处理和正常饲养的对照间的侵染死亡率无显著差异。

【Abstract】 ne hundred 4th instars of the Masson’s pine caterpillar were treatd withhigh(1×108 conidia/ml)and low(4×106)concentrations of Beauveria bassiana conidialsuspension,reared routinely,and weights of pine needles consumed, feces as well as each lar-va were recorded daily.The result showed that during the period from treating to the 15thday when death due to infection began,the needle consumption(Y)of each larva was corre- lated to the day(x)posttreatment in the following forms:for high concentration treatment,Y2=41.55-25.52x2+4.44x;for low concentration treatment,Y1=41.82-24.86x1+5.41x;for control,Y0=84.53-36.69x0+6.85x;Based on the above models,loss of pineneedles by the caterpillar was decreased due to Beattveria bassiana infection during the periodby 44.4% for the high concentration and 19.6% for low concentration.There was no sig- nificant difference between approximate digestibilities between the 2 treatments and the con- trol. Efficiencies of conversion of ingested fcod and effiencies of conversion of digested foodof the treatments were lower than those of the control,and once showed negative values,in-dicating that the infection did not affect the digestibility and a portion of food was convertedinto energy for severe defence reaction.The surviving larvae were reared and allowed to pupate for further development obser-vation.Pupation rates, emergence rates of the pupae as well as sex and weight of the pupaewere recorded.All the results indicated an obvious tendency of reduction as treatment con- centration increased.Male and female pupae were paired and allowed to emerge and depositeggs,The result showed a reduction of oogenesis as the concentration increased.Number offertilized eggs of the treatments and the control were estimated after the whole experimentcompleted.They were 1283,4826 and 9604 for high,low concentration and control,respec-tively;The actual increase rate of the experimental population were determined to be 12.83,48.26 and 96.04,respectively.Cosequently,reduction of increase rate of the caterpillar popu- lation by the high concentration treatment was estimated as(96.04-l2.83)/96.04×100%=86.6%and that by low 49.8%.In addition,Effect of starvation on fungal infection of the 4th instars was tested.Theywere sprayed with conidia of B. bassiazia,M.anisopliae and P,farinosus and then starved for 1,2,3 and 4 days before fed routinely.No significant difference was found between infec- tions of the treatments and non-starvation control.

【基金】 国家“八五”攻关项目
  • 【文献出处】 安徽农业大学学报 ,JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY , 编辑部邮箱 ,1996年03期
  • 【分类号】S763.420.6
  • 【被引频次】23
  • 【下载频次】106
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