【摘要】 应用显微外科技术剥除人胚肌腱外膜组织后，经过胰蛋白酶及胶原酶分步消化分离出人胚腱细胞，使用含２０％新生小牛血清的Ｆ－１２培养液将其传代培养１５代后全部冻存。该细胞贴壁生长，具有接触抑制的性质。细胞群体倍增时间大致为４天与细胞有丝分裂高峰时间相符。在传代培养过程中探索了体外培养的腱细胞生长的最适宜条件。通过冻存后复苏的细胞继续培养发现，冻存对其生长、形态、遗传学等特征无明显影响。本研究测定了培养细胞分泌羟脯氨酸量，提示培养的人胚腱细胞与体内腱细胞同样具有合成胶原的能力，并且这种能力不为传代所改变。更多还原

【Abstract】 Following the peritendon was removed by means of microsurgieal technique,the tenocytewas isolated from the human embryonic tendons by digesting it with trypsin and collagenase.These cells were all stored in frozen condition until they were cuItured by F-12 culture fluid addedwith 20%FBS to the 15th generation. These cells were able to grow adhering to the wall andstop growing with contact inhibition. The time of cellsgroup duplication was 4 days,which wassimilar to the peak time of its mitosis. The number of its chromosome group 2n=46 was 87.5-91.0%. The optimal conditions for tendon cell culture in vitro were investigated,and it wasfound that after they were reaminated and subcultured the frozen storage didn t influence theirgrowth,morphology,genetic characteristics. In our research we detected the content generation cells and found the cultured human em-bryonic tenocyte had same ability never changed with the cells subcultured.We also disscussedthe future of tenocyte-a biomaterial in the field of artificial implant.更多还原