【摘要】 用ＰＣＲ技术从我国水稻品种“广陆矮”（Ｏｒｙｚａ ｓａｔｉｖａ ｖａｒ． ｉｎｄｉｃａ）和“中花８ 号”（Ｏ． ｓａｔｉｖａｖａｒ． ｊａｐｏｎｉｃａ）中特异地扩增并克隆测序了富硫１０ ｋＤ醇溶谷蛋白基因，它共有５２５ 个核苷酸，编码１３４ 个氨基酸。经分析，克隆的基因与水稻属其它种或品种的同类基因同源率为９６．６％ 到１００％ ，与玉米１０ ｋＤ醇溶蛋白基因同源率为３４．２％ ；与某些双子叶植物的贮藏蛋白也有一定的同源性，例如和巴西豆富硫水溶蛋白同源率达３１．２％ 。水稻１０ ｋＤ醇溶谷蛋白Ｎ 端有一段信号肽含２４ 个氨基酸，经分析，发现这段信号肽与禾谷类玉米、高粱和燕麦的贮藏蛋白信号肽同源率很高，分别为６５．０％ 、６５．０％ 和６２．５％ ，而在双子叶植物的贮藏蛋白中未发现有相似序列。所测定的“广陆矮”和“中华８ 号”１０ ｋＤ醇溶谷蛋白基因序列已被ＥＭＢＬ数据库接受，收录号分别为Ｌ３６６０４ 和Ｌ３６６０５更多还原

【Abstract】 Using PCR technique, two prolamin genes from Oryza sativa var. indica (cv. Guangluai) and O. sativa var. japonica (cv. Zhonghua 8) were amplified and cloned. The prolamin gene contained 525 base pairs and encoded 134 amino acid residues. The two genes cloned from two different rice cultivars exhibited 100% homology and were highly homologous with the 10 kD prolamin gene in other rice species amountin an homology ranging from 96 6% to 100%. The deduced amino acid sequence shared 34 2% homology with that of maize 10 kD prolamin. As for dicots, only two types of storage protein shared some homology with rice 10 kD prolamin. One was from Brazil nut and the other from castor bean. Analysis on the signal peptide of rice 10 kD prolamin showed that it shared higher homology with that of storage proteins in some monocots such as maize, sorghum and oat. No similar sequence was found in dicots. The gene sequences of “Guangluai” and “Zhonghua 8” 10 kD prolamin would appear in EMBL data base under the accession number L36604 and L36605 respectively.更多还原