【摘要】 以１０８ 个玉米自交系随机组配成１９９ 个单杂交种为材料，用聚丙烯酰胺凝胶电泳（ＰＡＧＥ）检测过氧化物酶（ＰＯＤ）、细胞色素氧化酶（ＣＯＤ）、酯酶（Ｅｓｔ）、α－淀粉酶（α－Ａｍ ｙ）、过氧化氢酶（Ｃａｔ）、谷氨酸脱氢酶（ＧＤＨ）、苹果酸脱氢酶（ＭＤＨ）、超氧物歧化酶（ＳＯＤ）和酸性磷酸酯酶（Ａｃｐ）等同工酶及可溶性蛋白质的酶谱图式，探讨了酶谱差异指数与杂种产量优势的关系。结果发现，酶谱差异指数高的双亲杂交，能够产生高优势组合；酶谱差异指数低的双亲杂交，也能产生高优势组合；但酶谱差异指数极低的姐妹系杂交，只能产生低优势组合。由此证明，用酶谱差异指数方法来预测遗传背景复杂组合的杂种产量优势，在统计学上差异不太显著更多还原

【Abstract】 Electrophoretic isozyme zymogram patterns of peroxidase (POD), cytochrome oxidase (COD), esterase (Est), α amylase (α Amy), catalase (Cat), Glutamate dehydrogenase (GDH), Malate dehydrogenase (MDH), superoxide dismutase (SOD), acid phosphatase (Acp), etc. obtained from 108 maize inbred lines and their 199 hybrids were analyzed. The soluble protein patterns from these materials were tested as well. The authors had probed into the relationship between the index of zymogram difference and potential yield of heterosis in maize. The results indicated that hybrids from parents which showed high zymogram difference index could produce high heterosis and those from parents which had low zymogram difference index could also produce high heterosis as well. But hybrids from the sister lines which had lower zymogram difference index could only produce lower heterosis. There was no significant statistical difference between isozyme zymogram difference index and potential yield of heterosis on a genetic background with complex combinaton.更多还原