【摘要】 以白皮松（Ｐｉｎｕｓ ｂｕｎｇｅａｎａ Ｚｕｃｃ．）和油松（Ｐ．ｔａｂｕｌａｅｆｏｒｍ ｉｓ Ｃａｒｒ．）的未成熟胚乳，即雌配子体为外植体进行培养，将雌配子体分别接种在添加不同激素种类和不同浓度配比的改良ＭＳ培养基上诱导愈伤组织。经过２０多天的培养，在含有１—６ ｍ ｇ／Ｌ 萘氧乙酸（ＮＯＡ）和０．５ｍ ｇ／Ｌ６－ＢＡＰ及３％ 蔗糖浓度的培养基上诱导产生了愈伤组织，愈伤组织的诱导频率最高为２５％ 。经细胞学观察证明：愈伤组织细胞确为单倍性的，染色体数目为ｎ＝ １２，正常的体细胞染色体数目为２ｎ＝ ２４，并在含有ＡＢＡ 的原诱导愈伤组织培养基上分化出绿色小芽更多还原

【Abstract】 Immature endosperms (female gametophyte) of Pinus bungeana Zucc.and P.tabulaeformis Carr.were used as explants for establishing tissue cultures.Calli in duction and differentiation were studied on a modified MS medium containing 3% sucrose and various concentrations of auxins and cytokinins. Callus tissues of P.bungeana and P.tabulaeforms could be induced on media supplemented with 1—6 mg/L naphthoxyacetic acid and 0 5 mg/L 6 BAP . The highest induction frequency of calli was 25%. Histocytological observation revealed that the callus cell was haploidy with, N=12. Differentiation of green buds occurred on the medium supplemented with 0 1 mg/L ABA, whereas no plantlet was developed, however.更多还原