【摘要】 抗枯萎、抗黄萎病的棉种的浸取液，经硫酸铵分级，纤维素柱、亲和层析后，再经分子筛过滤，获得在ＰＡＧＥ、ＳＤＳ－ＰＡＧＥ或ＨＰＬＣ柱上均呈现单一蛋白带的棉花凝集素。该凝集素只凝集兔红细胞，对人Ａ、Ｂ或Ｏ型血细胞均不凝集，其凝集活性可被半乳糖或猪甲状腺球蛋白等所抑制。棉花凝集素在６５℃加热５ ｍ ｉｎ，即丧失全部凝集活性；它的凝集活性强烈地依赖于Ｃａ２＋ ；Ｍｎ２＋ 对活性也有促进作用，Ｍｇ２＋ 则无作用。经凝胶过滤或ＳＤＳ－ＰＡＧＥ测定，凝集素的分子量为６３０００，Ｎ－末端氨基酸为Ｖａｌ。凝集素含有１．５％ 的中性糖，是一种促有丝分裂原，细胞转化率为５０．３％ 。更多还原

【Abstract】 Defatted seeds of wilt disease resistant were extracted overnight with PBS at 4℃. After centrifugation, 90% saturated (NH 4) 2SO 4 was added to the supernatant. The precipitates were dialysed against H 2O, then lyophilized. The purified lectin was obtained by DEAE cellulose ion exchange chromatography, Sephadex G 100 filtration, and Sepharose 4B Hog thyroglobulin affinity chromatography. The activity of the lectin was tested with fresh rabbit erythrocyte in each step of the procedure, and the active part was collected. This sample demonstrated single band on PAGE, SDS PAGE and HPLC. The lectin was a glycoprotein. It contained 1.5% of neutral saccharide and its molecular weight was 63000 determined by Sephadex G 100 filtration. The N terminal amino acid of the lectin was Val. The lectin showed no specific agglutination with any type of human erythrocytes. The hemagglutinaition activity could be inhibited by galactose and hog thyroglobilin, and depended on Ca 2+ , Mn 2+ , especially on Ca 2+ , not Mg 2+ . Its biological activity lost at 65 ℃ for 5 min. This lectin is used as a mitogen for human peripheral blood lymphocytes.更多还原