【摘要】 从５１头临床病鹿中分离出３９株分枝杆菌（Ｍｙｃｏｂａｃｔｅｒｉｕｍ），其中牛分枝杆菌（Ｍ．ｂｏｖｉｓ）与鸟－胞内分枝杆菌（Ｍ，ａｖｉｕｍ－ｉｎｔｒａｃｅｌｌｕｌａｒｅ）的分离率最高，分别为２５．０％和２０．５％。建立了以牛型ＰＰＤ为抗原的间接ＥＬＩＳＡ和变态反应ＯＴ点眼法两种诊断方法。两种诊断方法比较，结果有交叉，总符合率为８７．３８％。鹿对ＢＣＧ皮下接种有良好的免疫应答，最佳免疫程序为：仔鹿出生２４ｈ内第１次皮下免疫接种冻干ＢＣＧ０．７５ｍｇ；第２次免疫接种为１２月龄，方法同前；第３次免疫接种为２４月龄，方法同前。应用此免疫程序在５个结核污染场的３８０８头阴性鹿免疫试验证明，对仔鹿保护率为９９．７％，成鹿保护率为９９．９％。更多还原

【Abstract】 Thirty-nine strains of Mycobacterium were isolated from 51 deers thathad clinical symptom of tuberculosis. The isolation rate of the M. bovis and M.intracellulare were the highest among them,25.0%and 20.5%respectively. Two diag-nostic methods had been established:one was indirect ELISA in which bovine-type PPDused as antigen,the other was ophthalminc allergic reaction used OT as allergin. Ac-cording to the comparision of the two diagnostic methods,crossreaction occured. The a-greement rate was 87. 35%. The deers could produce good immune response to BCGgiven subcutaneously. The best immunization precedure was that the deers were firstvaccinated in 24 h after birth with 0.75 mg BCG given subcutaneously,and boosteredtwice at the age of 12 months and 24 months with the same method. Using this immu-nization precedure to immunize allergic reaction negative deers from 5 deer farms infec-ed with deer tuberculosis,it was shown that the protective rate was 99.7%for newborndeers, 99.9%for adult deers.更多还原