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单克隆抗体胶乳凝集试验检测囊虫病循环抗原
Detection of Circulating Antigen in Cysticercosis by Latex Agglutination Test with a Monoclonal Antibody
【摘要】 采用抗猪囊虫单克隆抗体化学交联胶乳免疫微球,分别检测了囊虫病患者脑脊液和血清中的循环抗原,阳性率分别为90%(27/30)和74.63%(50/67)。同时对比检测了脑脊液和血清中的囊虫抗体,结果表明,脑脊液的抗体检出率明显低于血清,循环抗原的检出率则无显著性差异。本实验方法检测循环抗原简便易行,有较高的特异性和敏感性,既可用于早期诊断和现症病人的确定,也可用于疗效考核。
【Abstract】 The cerebrospinal fluids and sera from the patients with cysticercosis were tested with immunolatex spheres conjugated with a monoclonal antibody against cysticercus cellulosae. The detective rates of circulating antigen were 90% (27/30) and 74. 63% (50/67) respectively. The detective rate of the antibody in cerebrospinal fluids was evidently lower than that in sera but the differences of detective rates of circulating antigen were not apparent. Detection of circulating antigen in cysticercosis was useful for early diagnosis, active infection and evaluation of therapeutic effect.
【Key words】 Cysticercosis; Circulating antigen; Monoclonal antibody; Latex agglutination test;
- 【文献出处】 中国人兽共患病杂志 ,Chinese Journal of Zoonoses , 编辑部邮箱 ,1995年01期
- 【分类号】R446.6
- 【被引频次】4
- 【下载频次】19