节点文献
新的识别人活化B细胞分化抗原5C5单克隆抗体(5C5-G_1)的制备和鉴定
DEVELOPMENT AND IDENTIFICATION OF A NEW McAb 5C4-G1TO THE DIFFERENTIATION ANTIGEN 5C5 EXPRESSED ON HU- MAN ACTIVATED B LYMPHOCYTES
【摘要】 新制备的抗人活化B细胞分化抗原5C5单克隆抗体5C5-G_1经Wester印迹分析表明,该分化抗原经SDS-PAGE(还原条件下)显示五条带,分子量分别为92kD、52kD±(两条)、40kD和20kD。用单抗5C5和单抗5C5-G_1的混合物,从3D5细胞的λgtllcDNA文库中筛选到7个阳性cDNA克隆。
【Abstract】 A new McAb 5C5-G1 that reacts with the differentiation antigen 5C5 expressed on humanactivated B lymphocytes has been developed. McAb 5C5-G1 and McAb 5C5 develeped also in ourlaboratory recognize the same differentiation antigen. Western blotting shows five bands of theantigen with molecular weights of 92kD、52kD±、40kD and 20kD respectively. By using a mix-tures of McAb 5C5 and McAb 5C5-G1 for screening, 7 positive cDNA clones have been obtainedfrom λgtll cDNA library of a human activatd B cell line 3D5.
【关键词】 单抗5C5;
单抗5C5-G_1;
分化抗原5C5;
人活化B细胞;
【Key words】 McAb 5C5McAb 5C5-G1 Differentiation antigen 5C5 Activated human B cell;
【Key words】 McAb 5C5McAb 5C5-G1 Differentiation antigen 5C5 Activated human B cell;
- 【文献出处】 中国免疫学杂志 ,CHINESE JOURNAL OF IMMUNOLOGY , 编辑部邮箱 ,1995年05期
- 【分类号】R392
- 【被引频次】5
- 【下载频次】20