【摘要】 应用日本血吸虫单克隆抗独特型抗体ＮＰ３０腹腔注射致敏Ｃ５７ＢＬ／６小鼠，继之经脾脏注射虫卵形成的肝虫卵肉芽肿模型。用ＳＥＡ致敏组作阳性对照，ＳＰ２／０腹水组作阴性对照。实验结果表明，在虫卵攻击后第４ｄ，ＮＰ３０致敏组肝虫卵肉芽肿面积、体积即开始明显增加，出现多量嗜酸性粒细胞浸润。肉芽肿面积在第８ｄ、体积在第１５ｄ达高峰值，与ＳＥＡ致敏组的高峰值相近（Ｐ＞０．０５）。而ＳＰ２／０腹水组肉芽肿的面积、体积至第３２ｄ才达高峰值，其高峰值低于ＮＰ３０致敏组（面积Ｐ＜０．０５，体积Ｐ＜０．０１）。本文提示ＮＰ３０对肝虫卵肉芽肿的形成具有致敏作用。更多还原

【Abstract】 C57BL/6 mice sensitized intraperitoneally with the anti idiotypic monoclonal antibody NP30 of Schistosoma japonicum and then injected with vital egg suspension directly into spleen were used as the experimental models of hepatic egg granulomas in this study. The soluble egg antigen(SEA) sensitized group was used as positive control and the SP2/0 ascites injected group as negative control. The results showed that the size and volume of hepatic egg granulomas in the NP30 sensitized group increased obviously 4 days after egg injection and that a large number of eosinophiles appeared in egg granulomas. The size and volume of egg granulomas reached the peak values at d 8 and d 15 after egg injection, respectively. The peak values of the NP30 sensitized group were similar to those of the SEA sensitized group ( P >0.05). The size and volume of egg granulomas in the SP2/0 ascites injected group didnot reach the peak values, which were less than those of the group sensitized with NP30 (size∶ P <0.05; volume∶ P <0.01), until d 32 after egg injection. Our study suggests that NP30 may play a sensitizing role in the formation of hepatic egg granulomas.更多还原