【摘要】 以芜菁油菜为材料，从无菌苗５—８天叶龄的叶片分离叶肉原生质体，然后以６×１０￣４／ｍＬ的密度用ＭＰ１－３液体培养基进行浅层培养。培养１０天后，用ＭＰ４培养基对ＭＰ１－２中的培养物进行稀释（１：１）．用ＭＰ５培养基对ＭＰ３中的培养物进行稀释（１：１），每次间隔１０天。在培养期间时常用手轻轻摇动培养物。用这种方法，再生细胞分裂并形成愈伤组织，愈伤组织经ＭＳ１培养基增殖３周，转到分化培养基上诱导植株分化，分化出的苗经诱导生根发育成完整的小植株（共５４株）．在研究中发现：①激素的种类、浓度及组合对刺激细胞分裂的作用差异明显；②提高培养基的ｐＨ值可明显地控制细胞褐化；③ＡｇＮＯ＿３（５ｍｇ／ｍＬ）有益于愈伤组织分化植株；④谷氨酰胺（８０ｍｇ／ｍＬ）和腺嘌呤（４０ｍｇ／ｍＬ）能提高愈伤组织的植株分化频率；⑤原生质体培养基对原生质体植株的分化亦有影响．更多还原

【Abstract】 Using turnip rape as material,mesophyll protoplasts were isolated from 5-8 day-oldleaves of axenic shoots. Protoplasts were cultured in liquid media(MP1-3) as a thin layer with adensity of 6× 10￣4/mL. After 10 days of culture,the culture in MP1-2 were diluted with MP4 medi-um (1 :1),and the cultures in MP3 were diluted with MP5 medium(1: 1)at 10 day intervals.During protoplast culture,cultures were frequently shaken gently。 In this culture method,regener-ative cells divided and formed calli.Protoplast-derived microcalli were culture on the MS1 mediumfor 3 weeks nd then transfered onto the differentiative media(MS3-5)to induce shoot regenera-tibn.After 4 weeks on the dinerentiative media,shoots were formed from fhe calli.These shootswere induced to regenerated roots on MS6 mediurn and formed plantlets. Finally,the effects of cul-ture condition on protoplast culture and plantlet regeneration were discussed in the paper.更多还原