【摘要】 为了探讨白细胞介素（ＩＬ）－２转基因表达抗乙型肝炎病毒（ＨＢＶ）的作用，应用逆转录病毒载体ｐＺＩＰＳｖ（Ｘ）－包装细胞系ＰＡ３１７基因转移系统，研究了人ＩＬ－２ｃＤＮＡ转移和表达，以及抗ＨＢＶ和诱导淋巴因子激活的杀伤细胞（ＬＡＫ细胞）的作用。所构建的人ＩＬ－２重组表达载体ｐＺＩＰｈｕＩＬ－２，以ＤＮＡ－磷酸钙共沉淀技术转染ＰＡ３１７细胞，筛选到Ｇ４１８抗性克隆，分泌假病毒颗粒达１０６ＣＦＵ／ｍｌ。以假病毒颗粒感染２．２．１５细胞系及正常人外周血单个核细胞，分泌ＩＬ－２水平可达６ＩＵ／ｍｌ，明显抑制２．２．１５细胞ＨＢｓＡｇ的分泌表达，与１００ＩＵ／ｍｌ重组的ＩＬ－２一样可诱导出相似的ＬＡＫ细胞活性。而不含ＩＬ－２ｃＤＮＡ的ｐＺＩＰＳＶ（Ｘ）的假病毒颗粒则无此作用。提示逆转录病毒载体。包装细胞系可以成功地实现人ＩＬ－２ｃＤＮＡ的转移和低水平的分泌和表达，并可明显抑制２．２．１５细胞分泌ＨＢｓＡｇ及诱导ＬＡＫ细胞活性。更多还原

【Abstract】 sing molecular cloning techniques, we constructed and transfected human interleukinZ gene expressiveretroviral vector into a packaging cell line PA317. Ge-neticin resistant cell clones were identified and pseudovi-ral particles trtred as 3. 75 × 106 CFU/ml in their culturesupernatants. Human interleukin-2 expression level was5. 7llUlml for NIH3T3, 6IU/ml for both 2. 2. 15 andperipheral blood mononuclear cells after infection withpseudoviral particles with 8 of multiplicity of infection(M. O. IJ. Low level interleukin-2 expression poten-tially inhibited hepatitis B virus surface antigens expression of 2. 2. 15 cell line and enhanced cytotoxicity ofperipheral blcod mononuclear cells against K562 and Raji as target cells, but neither pseudoviral particles with-out interleukin-2 cDNA nor reccombinant interleukin-2at 6 IUlml. These results indicated that retroviral vec-torpackaging cell line can efficiently transfer and ex-press interleukin-2 cDNA in human somatic cells andlow level expression of interleukin-2 potentially inhibitshepatitis B virus surface antigens expression and enhances LAK activity of peripheral blood mononuclearcells of normal persons.更多还原