【摘要】 急性髓系白血病（ＡＭＬ）Ｍ＿（２ｂ）常伴有ｔ（８：２１）染色体易位，两个染色体重排导致形成ＡＭＬＩ／ＭＴＧ８融合基因，并持续产生融合基因转录本。作者利用逆转录。多聚酶链反应（ＲＴ－ＰＣＲ）扩增ＡＭＬ１／ＭＴＧ８融合基因转录本进行ＡＭＬ－Ｍ＿（２ｂ）微量残留白血病的监测，在１３例完全缓解的患者中，１２例可检出残留白血病细胞。经随诊这１２例中，２例已复发，１例死于强化治疗骨髓抑制期，９例仍持续完全缓解。更多还原

【Abstract】 cute myeloid leukemia （AML）-M_2b is frequently as-sociated with mutual translocation of chromosomes 8and 21 , t （8; 21） （q22; q22）. The rearrangement ofthese two chromosomes resulted in the formation ofAMLI/MTG 8 fusion gene and the production of a cor-respondent fusion transcript. The minimal residualleukemia were monitored by arnplification of AML1/MTG8 fusion mRNA using reverse transcription-poly-merase chain reaction （RT-PCR）. The fusion mRNAswere detected in 12 of 13 AML-M_2b patients in com-plete remission （CR） . Out of the 12 PCR positive pa-tients, two relapsed, one died of myelosuppression inintensification chemotherapy and nine remained in con-tinuous complete remission （CCR）. The only PCRnegative patient is also in CCR . Our preliminary re-sults suggested that residual leukemic cells carryingAMLllMTGS fusion gene could be detected by RT-PCR assay in most cases of AML-M_2b in CR, andseemed not to herald the impending relapse.更多还原