【摘要】 将分子生物学方法(聚合酶链反应和Digoxigenin标记的寡核苷酸探针打点杂交)、免疫学方法(单克隆抗体中和试验和蚀斑减数中和试验)及生物学方法(病毒Rct/40℃特征和D特征测定)相结合,用于Ⅰ型脊髓灰质炎病毒(PV)型内差异的综合鉴别诊断。分析比较了上述各方法区别野毒株与疫苗相关株,计算符合率的优缺点,为脊髓灰质炎病毒野毒株与疫苗相关株的鉴别诊断提供了依据。更多还原

【Abstract】 The polymerase chain reaction (PCR),dot-hybridization with digoxigenin-labelled probe,neutralization test with monoclonal antibodies, plaque reduction, neutralization test Rct/40C marker characterization assay and D. marker characterization assay were applied for the intratypic differentiation of type I polioviruses isolated in Wenzhou of China. We compared the accordance rates of intratypic differential on diagnosis by the methods above,and analyzed the advantages and disadvantages of the methods used for the diagnosis. This study is useful for the intratypic differentiation of wild and vaccine-associated strains of polioviruses in China.更多还原